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» Western Blot

» Immunofluorescence

Reactivity

• Bacteria, yeast, plant and mammalian cells
• GFP and its variants (EGFP and EBFP)

Excellent Quality

• Spontaneous fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria
• Transduces blue luminescence into green fluorescent light
• Ideal fluorescent probe in developmental and cell and molecular biology applications

High Specificity

• Fuses with proteins of interest without significantly interfering with their assembly or function
• No background staining / use at 1:5,000 - 1:10,000 dilution

Western Blotting Application:

Figure 1: Anti green fluorescent protein antibody: Cat # 210-PS-1GFP. Western blot analysis of whole cell lysates from cells infected with adenovirus coding for GFP (A and C) or GFP-PTG (protein-targeting to glycogen) fusion protein.
(A) 1 µg protein extract from primary culture hepatocytes from rat;
(B) 10 µg of protein extract from FTO2B cells;
(C) 10 µg of protein extract from HepG2 cells.

Image is courtesy of A. Adrover, S. Ros, D. Vílchez, D. Zafra and M. Garcia-Rocha. Barcelona Science Park. Spain.

 

Immunofluorescence Application:

The GFP antibody also works well for immunofluorescence:

Figure 2: Immunofluorescent staining of primary cultured hepatocytes from rat infected with a recombinant adenovirus coding for GFP. GFP auto-fluorescence (in green) and immunofluorescence with cat # 210-PS-1GFP (in red) at 1/300 dilution.

Image is courtesy of A. Adrover, S. Ros, D. Vílchez, D. Zafra and M. Garcia-Rocha. Barcelona Science Park. Spain.